Micro-Expression (ME) occurrence is only related to local region of face, with very short time and subtle movement intensity. There are also some unrelated muscle movements in the face during the occurrence of micro-expressions. By using existing global method of micro-expression recognition, the spatio-temporal patterns of these unrelated changes were extracted, thereby reducing the representation capability of feature vectors, and thus affecting the recognition performance. To solve this problem, the local region method was proposed to recognize micro-expression. Firstly, according to the region with the Action Units (AU) related to the micro-expression, seven local regions related to the micro-expression were partitioned by facial key coordinates. Then, the spatio-temporal patterns of these local regions were extracted and connected in series to form feature vectors for micro-expression recognition. The experimental results of leave-one-subject-out cross validation show that the micro-expression recognition accuracy of local region method is 9.878% higher than that of global region method. The analysis of the confusion matrix of each region's recognition result shows that the proposed method makes full use of the structural information of each local region of face, effectively eliminating the influence of unrelated regions of the micro-expression on the recognition performance, and its performance of micro-expression recognition can be significantly improved compared with the global region method.